MaghrebDataLibMedV1, Main, Exploration, bibRecord, 000633

Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.

Identifieur interne : 000633 ( Main/Exploration ); précédent : 000632; suivant : 000634

Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.

Auteurs : Salma Abdeljalil [Tunisie] ; Héla Trigui-Lahiani ; Houcine Lazzez ; Ali Gargouri

Source :

Molecular biotechnology [ 1559-0305 ] ; 2013.

RBID : pubmed:23242634

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Blotting, Southern (MeSH), Cloning, Molecular (MeSH), DNA, Fungal (genetics), Escherichia coli (genetics), Escherichia coli (metabolism), Fungal Proteins (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Library (MeSH), Genes, Fungal (MeSH), Models, Molecular (MeSH), Molecular Sequence Data (MeSH), Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Sequence Alignment (MeSH), Stachybotrys (enzymology), Stachybotrys (genetics), beta-Glucosidase (chemistry), beta-Glucosidase (genetics), beta-Glucosidase (metabolism).
MESH :
- chemical , chemistry : Fungal Proteins, Recombinant Proteins, beta-Glucosidase.
- chemical , genetics : DNA, Fungal, Fungal Proteins, Recombinant Proteins, beta-Glucosidase.
- enzymology : Stachybotrys.
- genetics : Escherichia coli, Stachybotrys.
- metabolism : Escherichia coli, Fungal Proteins, Recombinant Proteins, beta-Glucosidase.
- Amino Acid Sequence, Base Sequence, Blotting, Southern, Cloning, Molecular, Gene Library, Genes, Fungal, Models, Molecular, Molecular Sequence Data, Sequence Alignment.

Abstract

The filamentous fungus Stachybotrys microspora possess a rich β-glucosidase system composed of five β-glucosidases. Three of them were already purified to homogeneity and characterized. In order to isolate the β-glucosidase genes from S. microspora and study their regulation, a PCR strategy using consensus primers was used as a first step. This approach enabled the isolation of three different fragments of family 3 β-glucosidase gene. A representative genomic library was constructed and probed with one amplified fragment gene belonging to family 3 of β-glucosidase. After two rounds of hybridization, seven clones were obtained and the analysis of DNA plasmids leads to the isolation of one clone (CF3) with the largest insert of 7 kb. The regulatory region shows multiple TC-rich elements characteristic of constitutive promoter, explaining the expression of this gene under glucose condition, as shown by zymogram and RT-PCR analysis. The tertiary structure of the deduced amino acid sequence of Smbgl3 was predicted and has shown three conserved domains: an (α/β)8 triose phosphate isomerase (TIM) barrel, (α/β)5 sandwich, and fibronectin type III domain involved in protein thermostability. Zymogram analysis highlighted such thermostable character of this novel β-glucosidase.

DOI: 10.1007/s12033-012-9633-5
PubMed: 23242634

Affiliations:

Tunisie

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000637
to stream Main, to step Curation: 000637

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.</title>
<author><name sortKey="Abdeljalil, Salma" sort="Abdeljalil, Salma" uniqKey="Abdeljalil S" first="Salma" last="Abdeljalil">Salma Abdeljalil</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax, Tunisia. salma.abdeljalil@gmail.com</nlm:affiliation>
<country xml:lang="fr">Tunisie</country>
<wicri:regionArea>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax</wicri:regionArea>
<wicri:noRegion>3018 Sfax</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Héla" last="Trigui-Lahiani">Héla Trigui-Lahiani</name>
</author>
<author><name sortKey="Lazzez, Houcine" sort="Lazzez, Houcine" uniqKey="Lazzez H" first="Houcine" last="Lazzez">Houcine Lazzez</name>
</author>
<author><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:23242634</idno>
<idno type="pmid">23242634</idno>
<idno type="doi">10.1007/s12033-012-9633-5</idno>
<idno type="wicri:Area/Main/Corpus">000637</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000637</idno>
<idno type="wicri:Area/Main/Curation">000637</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000637</idno>
<idno type="wicri:Area/Main/Exploration">000637</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.</title>
<author><name sortKey="Abdeljalil, Salma" sort="Abdeljalil, Salma" uniqKey="Abdeljalil S" first="Salma" last="Abdeljalil">Salma Abdeljalil</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax, Tunisia. salma.abdeljalil@gmail.com</nlm:affiliation>
<country xml:lang="fr">Tunisie</country>
<wicri:regionArea>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax</wicri:regionArea>
<wicri:noRegion>3018 Sfax</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Héla" last="Trigui-Lahiani">Héla Trigui-Lahiani</name>
</author>
<author><name sortKey="Lazzez, Houcine" sort="Lazzez, Houcine" uniqKey="Lazzez H" first="Houcine" last="Lazzez">Houcine Lazzez</name>
</author>
<author><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name>
</author>
</analytic>
<series><title level="j">Molecular biotechnology</title>
<idno type="eISSN">1559-0305</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Blotting, Southern (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Fungal (genetics)</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Fungal Proteins (chemistry)</term>
<term>Fungal Proteins (genetics)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Gene Library (MeSH)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Models, Molecular (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Recombinant Proteins (chemistry)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Stachybotrys (enzymology)</term>
<term>Stachybotrys (genetics)</term>
<term>beta-Glucosidase (chemistry)</term>
<term>beta-Glucosidase (genetics)</term>
<term>beta-Glucosidase (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term>
<term>Alignement de séquences (MeSH)</term>
<term>Banque de gènes (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Escherichia coli (génétique)</term>
<term>Escherichia coli (métabolisme)</term>
<term>Gènes fongiques (MeSH)</term>
<term>Modèles moléculaires (MeSH)</term>
<term>Protéines fongiques (composition chimique)</term>
<term>Protéines fongiques (génétique)</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Protéines recombinantes (composition chimique)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Stachybotrys (enzymologie)</term>
<term>Stachybotrys (génétique)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Technique de Southern (MeSH)</term>
<term>bêta-Glucosidase (composition chimique)</term>
<term>bêta-Glucosidase (génétique)</term>
<term>bêta-Glucosidase (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term>
<term>Recombinant Proteins</term>
<term>beta-Glucosidase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term>
<term>Fungal Proteins</term>
<term>Recombinant Proteins</term>
<term>beta-Glucosidase</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines fongiques</term>
<term>Protéines recombinantes</term>
<term>bêta-Glucosidase</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Stachybotrys</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Stachybotrys</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
<term>Stachybotrys</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term>
<term>Escherichia coli</term>
<term>Protéines fongiques</term>
<term>Protéines recombinantes</term>
<term>Stachybotrys</term>
<term>bêta-Glucosidase</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term>
<term>Fungal Proteins</term>
<term>Recombinant Proteins</term>
<term>beta-Glucosidase</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term>
<term>Protéines fongiques</term>
<term>Protéines recombinantes</term>
<term>bêta-Glucosidase</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Blotting, Southern</term>
<term>Cloning, Molecular</term>
<term>Gene Library</term>
<term>Genes, Fungal</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Sequence Alignment</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Banque de gènes</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Gènes fongiques</term>
<term>Modèles moléculaires</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Technique de Southern</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The filamentous fungus Stachybotrys microspora possess a rich β-glucosidase system composed of five β-glucosidases. Three of them were already purified to homogeneity and characterized. In order to isolate the β-glucosidase genes from S. microspora and study their regulation, a PCR strategy using consensus primers was used as a first step. This approach enabled the isolation of three different fragments of family 3 β-glucosidase gene. A representative genomic library was constructed and probed with one amplified fragment gene belonging to family 3 of β-glucosidase. After two rounds of hybridization, seven clones were obtained and the analysis of DNA plasmids leads to the isolation of one clone (CF3) with the largest insert of 7 kb. The regulatory region shows multiple TC-rich elements characteristic of constitutive promoter, explaining the expression of this gene under glucose condition, as shown by zymogram and RT-PCR analysis. The tertiary structure of the deduced amino acid sequence of Smbgl3 was predicted and has shown three conserved domains: an (α/β)8 triose phosphate isomerase (TIM) barrel, (α/β)5 sandwich, and fibronectin type III domain involved in protein thermostability. Zymogram analysis highlighted such thermostable character of this novel β-glucosidase.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23242634</PMID>
<DateCompleted><Year>2013</Year>
<Month>11</Month>
<Day>04</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1559-0305</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>54</Volume>
<Issue>3</Issue>
<PubDate><Year>2013</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Molecular biotechnology</Title>
<ISOAbbreviation>Mol Biotechnol</ISOAbbreviation>
</Journal>
<ArticleTitle>Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.</ArticleTitle>
<Pagination><MedlinePgn>842-52</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s12033-012-9633-5</ELocationID>
<Abstract><AbstractText>The filamentous fungus Stachybotrys microspora possess a rich β-glucosidase system composed of five β-glucosidases. Three of them were already purified to homogeneity and characterized. In order to isolate the β-glucosidase genes from S. microspora and study their regulation, a PCR strategy using consensus primers was used as a first step. This approach enabled the isolation of three different fragments of family 3 β-glucosidase gene. A representative genomic library was constructed and probed with one amplified fragment gene belonging to family 3 of β-glucosidase. After two rounds of hybridization, seven clones were obtained and the analysis of DNA plasmids leads to the isolation of one clone (CF3) with the largest insert of 7 kb. The regulatory region shows multiple TC-rich elements characteristic of constitutive promoter, explaining the expression of this gene under glucose condition, as shown by zymogram and RT-PCR analysis. The tertiary structure of the deduced amino acid sequence of Smbgl3 was predicted and has shown three conserved domains: an (α/β)8 triose phosphate isomerase (TIM) barrel, (α/β)5 sandwich, and fibronectin type III domain involved in protein thermostability. Zymogram analysis highlighted such thermostable character of this novel β-glucosidase.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Abdeljalil</LastName>
<ForeName>Salma</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018 Sfax, Tunisia. salma.abdeljalil@gmail.com</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Trigui-Lahiani</LastName>
<ForeName>Héla</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lazzez</LastName>
<ForeName>Houcine</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gargouri</LastName>
<ForeName>Ali</ForeName>
<Initials>A</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Mol Biotechnol</MedlineTA>
<NlmUniqueID>9423533</NlmUniqueID>
<ISSNLinking>1073-6085</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.21</RegistryNumber>
<NameOfSubstance UI="D001617">beta-Glucosidase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013191" MajorTopicYN="N">Stachybotrys</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001617" MajorTopicYN="N">beta-Glucosidase</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year>
<Month>12</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2012</Year>
<Month>12</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2013</Year>
<Month>11</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">23242634</ArticleId>
<ArticleId IdType="doi">10.1007/s12033-012-9633-5</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Eukaryot Cell. 2004 Oct;3(5):1088-100</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15470237</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Genet. 2007 Jun;8(6):424-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17486122</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biosci Biotechnol Biochem. 1997 Jun;61(6):965-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9214755</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1995 Dec;15(12):7059-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8524273</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2006 Jan 15;22(2):195-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16301204</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1995 Dec 5;92 (25):11864-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8524864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Gen Genet. 1996 Dec 13;253(3):303-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9003317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 1982 Dec;44(6):1289-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16346147</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 1994 Dec;60(12):4387-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7811079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2004 Feb 27;13(4):573-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14992726</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2006 Jan 1;34(Database issue):D108-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16381825</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 1996 Sep;62(9):3165-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8795205</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Microb Physiol. 1995;37:1-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8540419</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Structure. 1999 Feb 15;7(2):179-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10368285</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem J. 2007 Dec 1;408(2):241-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17705786</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2011 Sep 29;8(10):785-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21959131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2006 Dec;73(4):807-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16896601</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2010 Apr 2;397(3):724-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20138890</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 1979 May;37(5):938-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16345389</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 1998 Oct;64(10):3607-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9758774</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2007 Nov;77(2):293-300</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17938914</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1973 Nov 15;80(4):575-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4204102</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2005 Dec 20;44(50):16529-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16342944</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Eng. 1997 Jan;10(1):1-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9051728</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Microbiol. 2003 May;179(5):339-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12640520</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Chem Biol. 2000 Oct;4(5):573-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11006547</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2010 Mar 12;11:166</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20222994</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1997 Sep 26;272(3):383-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9325098</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem J. 2010 Oct 1;431(1):39-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20662765</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 1995 Jun;61(6):2358-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7793956</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1331-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15213407</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Electrophoresis. 1997 Dec;18(15):2714-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9504803</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2002 May;14(5):1033-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12034895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem J. 1991 Dec 1;280 ( Pt 2):309-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1747104</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18838391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1984 Oct 10;259(19):11901-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6434532</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1993 Sep 28;32(38):9906-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8399160</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gene. 2007 Feb 15;388(1-2):54-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17107764</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Biochem. 1978 Sep 15;90(1):191-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">101374</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2000 Feb 25;275(8):5817-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10681571</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Genet Genomics. 2001 Sep;266(1):56-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11589578</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Adv. 2000 Aug;18(5):355-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14538100</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Biochem. 1992 Oct 15;209(2):651-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1425671</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Tunisie</li>
</country>
</list>
<tree><noCountry><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name>
<name sortKey="Lazzez, Houcine" sort="Lazzez, Houcine" uniqKey="Lazzez H" first="Houcine" last="Lazzez">Houcine Lazzez</name>
<name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Héla" last="Trigui-Lahiani">Héla Trigui-Lahiani</name>
</noCountry>
<country name="Tunisie"><noRegion><name sortKey="Abdeljalil, Salma" sort="Abdeljalil, Salma" uniqKey="Abdeljalil S" first="Salma" last="Abdeljalil">Salma Abdeljalil</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MaghrebDataLibMedV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000633 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000633 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MaghrebDataLibMedV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:23242634
   |texte=   Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:23242634" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a MaghrebDataLibMedV1

This area was generated with Dilib version V0.6.38.
Data generation: Thu Jun 17 16:21:50 2021. Site generation: Thu Jun 17 21:51:18 2021

	Serveur sur les données et bibliothèques médicales au Maghreb
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur sur les données et bibliothèques médicales au Maghreb

Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.

Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki